#Flowjo software download software

#Flowjo software download software

After data is collected, the BD Accuri C6 software Zoom function allows for visualization of data at any scale, which allows for precise placement of gates and regions. Gating strategies and fluorescence compensation values can be set before, during, or after data collection. This results in a standardized fluorescence performance so that users do not need to adjust detector voltages.ĭata is collected over 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. The laser and optical alignments are set and locked down during the manufacturing of the instrument. The Accuri is equipped with two 20mW lasers (488nm and 552nm) and 6 parameters for detection, which include 2 light scatter parameters (forward scatter and side scatter) and 4 parameters for acquiring fluorescence. To schedule Assisted Use or Training please contact Richard Salinas.įees: Trained user: $38/hour. Assisted use: $98/hour BD Accuri SORP Flow Cytometer Instrument Access: Assisted use and training is available. Instrument Configuration: Fortessa Configįluorescence Spectrum Analyzer (BD Biosciences) The Fortessa only accepts 5mL polystyrene tubes, or 96- and 384-multi-well plates if using the HTS unit. Sample analysis can also be done using Flowjo or FCS Express (both available to use in NMS2.114).

Sample acquisition and analysis is done using FACSDiva v6.1.3. In addition, this system is also equipped with the FACSFlow Supply System to allow larger volume tanks for sheath and waste, as well as improved fluidic stability. This instrument can be connected to the optional High Throughput Sampler (HTS) unit to accommodate automated specimen handling for 96- and 384-well plates. The Fortessa is equipped with four lasers (405nm, 488nm, 561nm and 635nm) and 15 parameters for detection, which includes 2 light scatter parameters (forward and side scatter) and 13 fluorescence parameters.